Subsections
[Cr:4, Lc:0, Tt:0, Lb:12]
- Introduction to molecular biophysics and spectroscopic techniques in
biology (2 Lectures) Determination of the critical micellar
concentration of a biological surfactant (bile salt) using
fluorescence spectroscopy. (1 Lab session) Basic structural
characterizations of a model protein using circular dichroism,
fluorescence, Raman spectroscopy etc. (3 Lab sessions) Urea and pH
denaturations of a model protein using circular dichroism and
fluorescence spectroscopy. (2 Lab sessions) Thermal denaturation of a
model protein using circular dichroism spectroscopy. (1 Lab session)
Fluorescence correlation spectroscopy experiment to measure the
protein diffusion and hydrodynamic size. (2 Lab sessions) Atomic force
microscopy of plasmid DNA. (2 Lab sessions)
- K. E. van Holde, C. Johnson, P. S. Ho Principles of Physical
Biochemistry, 2nd Edn., Prentice Hall (2005).
- C. R. Cantor and P. R. Schimmel, Biophysical Chemistry (Part
1-3), 2nd Edn., W. H. Freeman (2009).
