Subsections
[Cr:4, Lc:1, Tt:0, Lb:9]
- UV-Visible spectrophotometry: (a) Determination of concentrations of
absorbing, and non-absorbing species using chromophoric reagents (such
as the use of 1,10-phenanthroline for iron); (b) Method of continuous
variation for determination of stoichiometry of complexes.
- Electrochemical methods: (a) Potentiometry-estimation of chloride and
iodide in a mixture; use of ion-selective electrodes for the
determination of chloride and fluoride – determination of fluoride in
drinking water; (b) Electrogravimetry: Determination of Cu and Pb in
brass, or determination of purity of a Cu piece; (c) Voltammetry and
coulometry (may be included); (d) Fabrication of a screen printed
SnO
sensor for the detection and measurement
of NH
.
- Separation techniques: (a) GC – separation of a mixture of organics f
ollowed by identification using IR; (b) HPLC-separation of organics
and comparison with GC, (c) separation of lanthanides using HPLC.
- Fluorescence spectroscopy: (a) Determination of fluorophore
concentrations using emission and synchronous fluorescence methods.
(b) Study of ligand sensitized fluorescence; (c) Effects of quenching
– Stern Volmer equation.
- Microscopy: Raman Microscopy and AFM
- Atomic Absorption and Emission Spectroscopy – Preparation of
standards at
g/mL concentrations (ppm) and use in the determination
of trace levels using Atomic Absorption and Atomic Emission
techniques, using flame and electrothermal vaporization techniques
- Determination of Ca, Mg and Cu in tap water, and waste water samples
by Flame Atomic Absorption Spectroscopy
- Determination of total organic carbon chemical oxygen demand in water
samples
Notes
- The lab course has been designed to accommodate a total of about 20
experiments. A student may be required to do about 15 of these
experiments in the course.
- If AAS spectrometer and GC-MS are not available, the course may be
conducted using experiments listed from 1-5, instruments for which are
available or would become available, and which would account for 15
experiments.
- A. Ghosal, B. Mahapartra, A. K. Nad, An Advanced Course in
Practical Chemistry, New Central Book Agency Pvt Ltd, Calcutta
(2000).
- D. A. Skoog, F. J. Holler, S. R. Crouch,: Principles of
Instrumental Analysis, 6th Ed, Brooks Cole, USA (2006).
