Subsections
[Cr:4, Lc:4, Tt:0, Lb:0]
- Organization of the eukaryotic genome. Nucleosomes, histones,
chromatids, centromeres, teleomeres. Repetitive content of eukaryotic
genomes. Organization of prokaryotic genomes. Organization of genes,
operons.
- Mapping of Genomes. Genetic maps. DNA markers: RFLP, SSR, SNP,.
Linkage analysis as a basis of genetic analysis. Physical maps.
Restriction maps, FISH, STS mapping
- Sequencing of genomes. Methods of DNA sequencing. Maxam and Gilbert
method. Sanger method. Automated DNA sequencing. Pyrosequencing. An
introduction to Next Generation sequencing strategies. Genome
sequencing strategies. Assembly of contiguous DNA by hierarchial (
clone contig) and shotgun strategies. BACs, YACs in genome sequencing
strategies.
- The Human genome project and the history of genome sequencing. The
mapping phase. Sequencing the human genome. Future implications and
impact. Genome sequencing projects of model organisms.
- How genomes function: Patterns of DNA methylation in plants and animals.
Chromatin modification and genome expression. Histone acetylation, deacetylation,
nucleosome remodelling. Role of histone substitution and histone variants in gene regulation.
Maintainence and transgenerational inheritance of epigenetic marks.
RNA-dependent DNA methylation (RdDM) pathway in transcriptional gene silencing (TGS).
- Experimental approaches to genome-wide analysis. Genome-wide
mutational strategies. Transcriptome analysis using microarray. ChIP
on ChIP assays.
- Genome evolution and comparative genomics. Genome duplication,
horizontal gene transfer. Orthologues,paralogues. Horizontal gene
transfer. Organellar evolution.
- T. A. Brown, Genomes 3, 3rd Edn., Garland Science (2006).
- D. L. Hartl and E. W. Jones, Genetics: Analysis of Genes and
Genomes, 7th Edn., Jones and Bartlett Publishers (2009).
