Subsections
[Cr:4, Lc:3, Tt:0, Lb:0]
- Background Concepts: Protein structure and function; Protein folding and misfolding;
Protein stability (enzymatic, thermodynamic and kinetic).
- Objectives, Concepts and Approaches: Creation of new or altered structural proteins,
catalysts, transporters, receptors and transcription factors; Redesign of structure,
function, stability and aggregation; Creation of designer proteins; Combinatorial
approaches to protein engineering; Rational approaches to protein engineering.
- Tools: Molecular visualization; Molecular modelling; Recombinant DNA technology-
based strategies; Constructs, vectors, strains, affinity fusion tags; Chemical modification
strategies; Use of labels; Heterologous protein expression; Protein purification
approaches; Protein handling; Protein biochemical and physico-chemical
characterization; Design and development concepts relating to functional assays
- Combinatorial Protein Engineering: Chemical mutagenesis; Error-prone PCR; Synthetic
peptide combinatorial libraries; Principles and applications of phage display and
bacterial display; Selection and screening approaches for folding and function
- Rational Protein Engineering: Backbone Reversal; Domain or sub-structure fusion;
Global conservative mutagenesis; Excision of super-secondary structures; Substructure
shuffling with symmetric structures; Surface reengineering to obviate aggregation;
Disulphide bond introduction to prevent domain dissociation; Topology scrambling
through loop redesign; Active surface transplants between homologous beta sheet
proteins; Whole surface transplants; Enzyme active site transplants; Loop transplants on
beta/alpha barrels; Site- directed mutagenesis to alter protein kinetic stability or
thermodynamic stability; Protein hydrophobic core reengineering; Reengineering of
protein surface electrostatics.
- Sheldon J. Park & Jennifer R. Cochran (Eds) Protein
Engineering and Design, CRC
Press (2009).
- Mallorie N. Sheehan Protein Engineering: Design,
Selection, and Applications, Nova
Science Publishers, Inc. (2011).
- Jeffrey L. Cleland & Charles S. Craik (Eds), Protein
Engineering: Principles and
Practice, Wiley (1996).
