Subsections
[Cr:4, Lc:3, Tt:0, Lb:0]
- Introduction to Synthetic Biology, Bacterial Promoters and Prokaryotic Gene regulation, Early Synthetic Biology Experiments based on gene regulation, Orthogonality of parts- context independance.
- Quantitative representation of gene regulation, Negative feedback loops Positive feedback loops,
Simple synthetic networks, Reprissilator, Toggle switch Networks; Scale-free networks, Gene regulatory networks
Response times; Robustness and Stochasticity.
- Directed Evolution in synthetic biology, Methods of generating diversity; Screening and selection in directed evolution.
- Allostery and Allosteric Switches, Engineering allostery: Protein Fusions and linkers,
Combinatorial approaches, Domain insertions, Engineering GFP to make sensors.
- Oligonucleotide synthesis, Gene synthesis, Genome synthesis, Genome Assembly, Synthetic Genomes
Synthetic Biology approach to Minimal Genomes, Extension of the Genetic code.
- Metabolic networks and Metabolic Engineering, Metabolic Flux analysis, 13C- Metabolic Flux Analysis
Metabolic engineering for synthetic Biology.
- Recent Applications of Synthetic Biology, Quorum sensing and RNA riboswitches as a tool iGEM competitions.
- H. Zhao, Synthetic Biology: Tools and Applications, Academic Press, 2013.
- Campbell, A. M. and Heyer L., Discovering Genomics, Proteomics and Bioinformatics, Pearson, 2007.
- Voigt, C. Synthetic Biology, Part A, Methods in Enzymology, V 497, Academic Press, 2015.
- Voigt, C. Synthetic Biology, Part B, Methods in Enzymology, Vol, 498, Academic Press,2015.
- Gibson, D. G. et.al. Editors. Synthetic Biology: Tools for Engineering Biological Systems, Cold Spring Harbor Press, 2017.
