Subsections
[Cr:4, Lc:3, Tt:0, Lb:1]
- Part I: Purification and isolation and identification of immunoglobulins and their sub-units, Proteolytic cleavage of lg molecules by enzymes and their usage. Various serological tests (precipitation, aggluti-
nation reaction-based tests), bedside or point-of-care immunodiagnostic tests, Immunoassays (ELISA,
radioimmunoassay, Fluoroimmunoassay, immunohistochemistry, ELISPOT assay et ), The use of flow cytometry in disease diagnosis and monitoring of treatment efficacy.
- Part II: Separation and enumeration of lymphocytes by conventional and flowcytometry based methods:
Culturing of myeloid and lymphoid cells and determining their functional ability, In vitro and in vivo
tests for phagocytosis, Apoptotic changes in lymphoid and non-lymphoid cells.
- Part III: Traditional and modern methods of production of antibodies: such as animal immunization,
hybridoma and rDNA technologies including antibody display technologies, Multicolor flowcytometric
analyses of lymphocytes and other cells, In vitro differentiation of helper T cells, Identifying immuno-
genic epitopes of intracellular pathogens. Genesis and use of transgenics, transnuclear as well as
Knock-in and knockout mice models for immunological investigations.
- Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM & Strober W., Current Protocols in Immunology, 3rd Ed., John Wiley & Sons. 2003, (subsequent versions)
- Detrick B & Hamilton RG. (Eds)., Manual of Molecular and Clinical Laboratory Immunology, 7th Ed.
American Society for Microbiology, 2006.
- Hay FC & Westwood OMR,
- Practical Immunology, 4th Ed. Blackwell, 2002.
